Identification of the major tamoxifen-DNA adducts in rat liver by mass spectroscopy.
نویسندگان
چکیده
We present here the first mass spectroscopic (MS) identification of the main tamoxifen-induced DNA adducts in rat liver. The two main adducts were isolated by high-performance liquid chromatography (HPLC) and identified by MS, MS-MS and ultraviolet spectroscopy. Adduct 1 was the N-desmethyltamoxifen-deoxyguanosine adduct in which the alpha-position of the metabolite N-desmethyltamoxifen is linked covalently to the amino group of deoxyguanosine. Adduct 2 was confirmed to be the trans isomer of alpha-(N2-deoxyguanosinyl)tamoxifen, as previously suggested by co-chromatography.
منابع مشابه
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عنوان ژورنال:
- Carcinogenesis
دوره 20 2 شماره
صفحات -
تاریخ انتشار 1999